Laboratory Technician for EV biomarkers research

Profile

Overall Position Summary and Objectives
The purpose of this position is to support research inside our laboratory regarding biomarker studies using Extracellular Vesicles (EVs) in large longitudinal study cohorts. The incumbent will conduct isolation of EVs from human (and potentially rodent) plasma and other biofluids using appropriate techniques. In addition, will conduct characterization of EVs by Nanoparticle Tracking Analysis, Light Microscopy, Western Blotting, and other techniques. In addition, will conduct ELISA-type immunoassays using various platforms (ELISA kits, Luminex, Mesoscale, SIMOA, etc.); will perform QC assessments; assemble data in spreadsheets; assist in analyzing and interpreting data; assist in preparing graphs, figures and manuscripts.
Minimum Education
Bachelor's
Certifications & Licenses
Good Laboratory Practice (GLP)
Skills (Ranked By Priority)
Extracellular Vesicle isolation
Extracellular Vesicle characterization
ELISA and immunoassays
Western Blots
Cell cultures
Software
PowerPoint
WORD
EXCEL
Statistic packages (such as R, SPSS)
client POTS
Outlook
Field of Study
Neuroscience
Molecular Biology
Miscellaneous Biology
DELIVERABLES Conduct EV isolation using established laboratory protocols in plasma/serum samples from large cohorts, including but not limited to CHARIOT-PRO, HABS, BLSA and University of Basel cohort. Conduct immunoassays, including ELISA, Luminex, Mesoscale, SIMOA, etc. Characterize EVs by NTA, WB and other techniques. Record and organize data in spreadsheets. Prepare graphs, figures and tables based on data. Conduct statistical analysis. Interpret results and contribute to writing of publications. - Monthly
Inspect Equipment - Weekly
Meet with lab members to present updates - Bi-Weekly
STATEMENT OF WORK DETAILS
Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis
Follow established procedures and protocols; utilize aseptic technique to perform experiments
Perform a variety of laboratory techniques and procedures
Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence immunohistochemistry
Perform experiments on samples according to protocols
functional responsibility
Operates laboratory machines and instruments; and prepares specimens and equipment for use.
Maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents and staining
Process and organize multiple samples simultaneously
Work with a variety of routine and specialized laboratory instruments and equipment in the laboratory
Ensure that all specimens and equipment necessary for experiments are available and in good order
Perform processing, description and analysis of specimens
functional responsibility
Performs sample management and storage.
Store new samples, update database, retrieve samples with proper authorization
Upkeep sample and database inventory; monitor overall sample inventory
Retrieve, catalog and process samples including but not limited to blood and tumor tissue
Perform quality control procedures necessary to ensure the accuracy of each test system, sample processing, storage and inventory of samples
Perform processing, description and analysis of samples
functional responsibility
Analyzes, documents and reports on all procedures performed.
Convey and discuss research results with team lab members
Update and maintain written laboratory record of the work
Collect and enter research data into laboratory notebooks and databases
Collect data and prepare for analysis and interpretation
Analyze, summarize and interpret data obtained from research studies; evaluate for completeness and validity
Draw conclusions about the results as related to the ability of the experiments to answer the questions posed
functional responsibility
Maintains inventory of laboratory supplies.
Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory
functional responsibility
Cleans and sterilizes instruments, glassware and equipment.
Work with staff to cover glassware washing, media preparation, and autoclaving
Clean and sterilize laboratory equipment such as glasses, metal instruments, sinks, tables and test panels
functional responsibility
Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
Perform primary cell culture techniques and immunohistochemistry
Follow established procedures and protocols; utilize aseptic technique to perform experiments
Perform a variety of laboratory techniques and procedures
Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence immunohistochemistry
Perform experiments on samples according to protocols
functional responsibility
Maintains inventory of laboratory supplies and initiates purchase orders.
Monitor and inventory routine laboratory supplies; ensure that supplies are stocked and organized
Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory
Establish and maintain a record of laboratory supply usage
Inventory laboratory supplies and equipment, make recommendations and prepare purchase requests
Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results
functional responsibility
Performs routine equipment maintenance.
Perform laboratory maintenance tasks, including scheduling liquid waste container pickup
Monitor equipment performance and maintenance schedules
Assist in troubleshooting and calibrating various laboratory equipment and instruments
Coordinate general laboratory maintenance, including equipment upkeep and calibration
Perform preventive maintenance on laboratory equipment
functional responsibility
Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis
Perform primary cell culture techniques and immunohistochemistry
Follow established procedures and protocols; utilize aseptic technique to perform experiments
Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence immunohistochemistry
Perform experiments on samples according to protocols
functional responsibility
Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff.
Provide experimental progress updates on procedures performed in the laboratory
Prepare reports and summaries based on results and findings
Record details of experimental procedures, results and protocols
Present results and conclusions in written, tabular and graphic form
Prepare accumulated data and conclusions for publication in professional journals
Collect, analyze and interpret data; prepare summaries and reports based on results and findings
functional responsibility
Performs advanced equipment maintenance.
Assist in troubleshooting and calibrating various laboratory equipment and instruments
Coordinate general laboratory maintenance, including equipment upkeep and calibration
Perform regularly scheduled maintenance on and generate maintenance reports for equipment
Perform preventive maintenance on laboratory equipment
Carry out calibration; organize and initiate service and preventative maintenance on equipment
functional responsibility
Trains staff in performing routine laboratory tests and procedures.
Provide technical help and expertise to lab members
Provide training to staff on the performance of laboratory experiments, assays and protocols
Train new staff on the use, calibration and maintenance of laboratory equipment
Train new laboratory members in the correct use of equipment, application of techniques and laboratory protocols
Work with staff to monitor and ensure that appropriate laboratory safety courses and certifications for staff are up to date
functional responsibility
Provides insights and expertise and recommends new strategies and approaches.
Engage in problem solving as required to solve technical difficulties and to ensure steady progress, with a goal of providing high quality experimental data suitable for publication
Evaluate new types of experimental approaches analyses and recommend changes to protocols
Determine when modifications and adjustments are necessary for experiments
Identify pitfalls in the original choice of methods or experimental direction and redirect the study to achieve improved results
Troubleshoot issues with technical procedures; utilize trial and error methods customize protocols to the biological systems utilized in the laboratory
22nd Century Technologies is an Equal Opportunity Employer and US Citizens & all other parties authorized to work in the US are encouraged to apply. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability or protected veteran status.

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